Coding

Part:BBa_K4769220

Designed by: Kieran Abbott   Group: iGEM23_Cambridge   (2023-10-10)


Porphyran utilisation locus fragment 3

Fragment 3 of the porphyran utilisation locus; the porphyran utilisation locus has been identified to provide the ability to digest the polysaccharide, porphyran, found in seaweed (see DOI: 10.1038/s41586-018-0092-4)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 595
    Illegal EcoRI site found at 4174
    Illegal PstI site found at 1712
    Illegal PstI site found at 1895
    Illegal PstI site found at 2794
    Illegal PstI site found at 5603
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 595
    Illegal EcoRI site found at 4174
    Illegal PstI site found at 1712
    Illegal PstI site found at 1895
    Illegal PstI site found at 2794
    Illegal PstI site found at 5603
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 595
    Illegal EcoRI site found at 4174
    Illegal BglII site found at 1490
    Illegal BglII site found at 2011
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 595
    Illegal EcoRI site found at 4174
    Illegal PstI site found at 1712
    Illegal PstI site found at 1895
    Illegal PstI site found at 2794
    Illegal PstI site found at 5603
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 595
    Illegal EcoRI site found at 4174
    Illegal PstI site found at 1712
    Illegal PstI site found at 1895
    Illegal PstI site found at 2794
    Illegal PstI site found at 5603
    Illegal NgoMIV site found at 2785
    Illegal AgeI site found at 1351
    Illegal AgeI site found at 2573
    Illegal AgeI site found at 3226
    Illegal AgeI site found at 6301
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 4810


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Categories
Parameters
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